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PubMed provides access to bibliographic information, drawn primarily from MEDLINE and PREMEDLINE.

PubMed also provides access to the molecular biology databases included in NCBI's Entrez retrieval system. MEDLINE is the National Library of Medicine's (NLM) premier bibliographic database covering the fields of medicine, nursing, dentistry, veterinary medicine, the health care system, and the preclinical sciences. The MEDLINE file contains bibliographic citations and author abstracts from approximately 3,900 current biomedical journals published in the United States and 70 foreign countries. The file contains approximately 9 million records dating back to 1966.

As of August 1996, NLM introduced the PREMEDLINE database which provides basic citation information and abstracts before the full records are prepared and added to MEDLINE. New records are added daily, the completed records are added weekly to MEDLINE, and deleted from the interim PREMEDLINE database.